Glucose Transporter 8 Positive

In vitro Assessment of Transporter-Mediated Uptake in Plated Cryopreserved Hepatocytes

实验设备   37°C water bath 37°C / 5% CO2 humidified incubator Orbital shaker placed inside incubator 实验步骤   1. Prepare compound stocks: test articles (TA) and positive control(s) (PC) dissolved

Assessment of Drug Transporter Function Using Fluorescent Cell Imaging

, appropriate to the transporter, e.g., Rhodamine 123 (Sigma‐Aldrich) or Hoechst 33342 (Sigma‐Aldrich) Positive control inhibitor, appropriate to the transporter, e.g., PSC833 (Xenotech) or Ko143

做原核表达的几点教训和体会

： 从左到右的泳道都是按a, b, c, d处理排列的。5－8以及13－16泳道是positive expression control。是pET15b携带b-galactosidase的一个构建。泳道1－8是全细胞蛋白电泳；泳道9－12是取沉淀电泳；13－16是上清电泳。结果： a. 有glucose的阴性对照无带（或非常浅） b. 无glucose的阴性对照有带，但整体来说比诱导的弱 c. 诱导的有带 d. 蛋白大小还是不对（其实是对的，我冤枉它了，请看