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文献和实验Analyses of PCSK9 Post-translational Modifications Using Time-of-Flight Mass Spectrometry
successfully for protein/peptide profiling in disease states and for the detection of protein/peptide biomarkers (1–4). In this chapter, the secreted proprotein convertase subtilisin/kexin 9 (PCSK9), which we study in our lab, is used to demonstrate coupling
Utilizing Peptide SPOT Arrays to Identify Protein Interactions
of synthesized peptides 12? to 18?amino acids long, with overlapping sequences that cover the entire sequence of a protein, covalently linked to a solid support. This unit describes how to construct peptide SPOT arrays, biotinylate recombinant proteins
Synthesis and Probing of Membrane-bound Peptide Arrays
Synthesis and Probing of Membrane-bound Peptide Arrays Ronald Frank Department of Chemical Biology, GBF (German Research Center for Biotechnology), 38124 Braunschweig, Germany
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