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文献和实验of conjugated-Ig is added to the column. If 2mL of conjugated-Ig is added, discard first 1mL and keep the next 4mL. The antibody is always diluted by 2x after leaving the column. 5. When FITC coupling an antibody, you can determine the FITC to Protein ratio
, you can determine the FITC to Protein ratio. When Biotinylating an antibody, you can test the antibody by Flow Cytometry. 6. F/P Ratio: a) Dilute an aliquot of FITC-Ig complex with buffer so that the Absorbance at 280nm (A280) is less than 2.0
Cell Death Differ:中大五院黄曦团队发现新冠病毒引起肺损伤的分子机制
病理发生。然而,SARS-CoV-2 感染引起肺细胞凋亡的机制仍不清楚。 2022 年 1 月 13 日,中山大学附属第五医院黄曦课题组在 Cell death & differentiation 上发表了题为 SARS-CoV-2 membrane protein causes the mitochondrial apoptosis and pulmonary edema via targeting BOK 研究性论文。该研究证实了病毒膜(M)蛋白会通过靶向 BOK 分子,介导肺
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