赛默飞世尔Thermo Fisher 实验室台端计时器 1E

核酸电泳工作流程——5 大主要步骤

), Nucleic Acid Electrophoresis (Springer Lab Manual). Heidelberg: Springer. pp 1–53. 2.Thermo Fisher Scientific Inc (2010) Nucleic Acid Detection and Analysis. In: Molecular Probes Handbook: A Guide to Fluorescent Probes and Labeling Technologies. pp 349–360

SDS-PAGE

like the Biorad model 1000/500 you can do a run like this automatically (without having to be in lab to switch up the Amps in the middle or shut them off at the end). Alternatively, for overnight runs, run the gel at 11 mAmps and set the timer to turn the gel

小RNA的Northern BLOT

#______________  1 μl T4 Polynucleotide Kinase, 10 U/μl Lot#______________  X μl Nuclease free H2O to 10 μl  37℃ at least 1 hr  After incubation, bring reaction volume up to 50 μl before cleaning up with spin column