赛默飞世尔Thermo Fisher 矩形瓶 BOTTLE

【转贴】immunofluorescence staining

Scientific, Cat. #6663-F10, #1 thickness 11 X 22 mm rectangular coverslips.22 X 22 mm coverslips may also be used, but will require larger volumes of reagents:Fisher Scientific, Cat. #12-520-B, #1 1/2 thickness 22 X 22 mm square coverslips.Note

Procedures for In Vitro Production of Bovine Embryos

all debris behind (repeat twice to assure that oocytes are clean of debris; Figure 10).Figure 10. An X-plate used to wash COCs, purchased from Fisher .Figure 11. Washing of COCs using an X-plate.Figure 12. Some instruments used to pick up embryos

小RNA的Northern BLOT

#______________  1 μl T4 Polynucleotide Kinase, 10 U/μl Lot#______________  X μl Nuclease free H2O to 10 μl  37℃ at least 1 hr  After incubation, bring reaction volume up to 50 μl before cleaning up with spin column