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- 详细信息
- 文献和实验
- 技术资料
- 库存:
9999
- 英文名:
Lysing Matrix E, 2 mL tube
- 供应商:
广州威佳科技有限公司
- 规格:
100x2mLtubes
Product Description
2 mL裂解介质E内含1.4 mm陶瓷珠、0.1 mm硅珠和一颗4 mm玻璃珠。裂解介质E是紫色管盖。
Application Notes
裂解介质E对于混合样本也很有效,如微生物感染的组织,并且可以从肿瘤组织或者其他困难样本中提取RNA。
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
核酸分离|核酸提取|蛋白质组学|纯化和检测|蛋白质纯化|DNA纯化|RNA纯化|环境样本分析|土壤分析|粪便样本裂解|粪便基因组学研究|水体分析|污泥分析|微生物组学研究|肿瘤均质化|代谢产物提取|样本均质化
Specifications
| SKU | 116914100 |
| Alternate Names | E Matrix |
| Application Notes | 裂解介质E对于混合样本也很有效,如微生物感染的组织,并且可以从肿瘤组织或者其他困难样本中提取RNA。 |
| Average Size | Ceramic Spheres, 1.2 - 1.6 mm, Silica Spheres, 0.074 - 0.150 mm, Glass Bead, 4 mm |
| Base Catalog Number | 6914050 |
| Capacity | 2 mL |
| FastPrep Instruments Models | ConeFlex Legacy (allows all FP24 adapters on FP96), CoolPrep (24x2 mL), HiPrep (48x2 mL), QuickFlex (96x2 mL), QuickPrep (24x2 mL), QuickPrep All Metal (24x2 mL) |
| Format | 2 mL tube |
| Hardness | Ceramic Spheres: Vickers 800, Silica Spheres: Knoop 515, Glass Bead: Knoop 515 |
| Molecular Formula | Ceramic Bead, ZrO2 64%, SiO2 33%, Glass Beads SiO2 |
| Personal Protective Equipment | If working with biological, toxic, hazardous or infectious samples use hand protection (Latex, Nitrile or other protective approved material gloves), skin and body protection (labcoat and long-sleeves) and eye protection (safety glasses with side-shields). Handle in accordance with good industrial hygiene and safety practices. |
| Sample Source | Environmental |
| Sample Type | Feces, Soil, Wastewater, Environmental Water, Sludge, Tumor |
| Sample Volume | up to 500 mg per prep |
| Specific Gravity | Ceramic Spheres: 4 |Silica Spheres: 2.5 |Glass Bead: 2.5 |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
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文献和实验蛋白溶酶原转变成纤维蛋白溶酶,后者通过如下三种反应影响炎症的进程:①通激活第Ⅻ因子启动缓激肽的生成过程;②裂解C 3 产生C 3 片断;③降解纤维蛋白产生其裂解产物,进而使血管通透性增加。 炎症介质的作用有两点值得注意。第一,不同介质系统相互之间有着密切的联系,例如图5-8所示的补体、激肽及凝血系统和纤维蛋白溶解系统的激活和其产物的密切关系,便是例证。这些炎症介质的作用也是交织在一起的。第二,几乎所有介质均处于灵敏的调控和平衡体系中。一方面在细胞
相关专题 成功走出第一步:DNA提取 碱裂解法 大量提取质粒(500mL菌液) 1、取培养至对数生长后期(一般培养12~16小时)的含待提质粒的细菌 培养液于离心杯中,配平,4℃下4000rpm离心15min,弃上清,然后将离心管倒置于干净的约纸上使上清液全部流尽。 2、向盛有细菌 沉淀物的离心管中加入20mL冰冷的溶液Ⅰ,重悬沉淀(先加5mL溶液Ⅰ,用干净的玻璃棒搅拌均匀后,再加剩下的部分)。
Purification of Plasmid from 50 ml-culture
1. Shake E. coli harboring plasmid at 37 C overnight in 50 ml of TB containing appropriate antibiotics. (when using ampicillin, addition of the antibiotics to 100-200 ug/ml rather than usual 50 ug/ml may improve the yield of plasmids.)
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