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- MP Biomedicals
- 116915100
- 2025年07月16日
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- 文献和实验
- 技术资料
- 库存：
9999
- 英文名：
Lysing Matrix F, 2 mL tube
- 供应商：
广州威佳科技有限公司
- 规格：
100x2mLtubes
Product Description
Lysing Matrix F, 2 mL Tube contains 1.6 mm aluminum oxide particles and 1.6 mm silicon carbide particles. Matrix F tubes have white caps. Will not bind nucleic acids.
Application Notes
Lysing Matrix F is effective at breaking tough, hard, or brittle cell membranes. This is useful in applications such as isolation of intact organelles, and super-molecular structures where lysis conditions necessitate high impaction but low shearing. Matrix F is effective for applications such as RNA and protein isolation for samples in which Matrix A is too agressive. The irregular, angular shape of the Al2O3 and SiC crystals give lower shearing than the sharp edges of the garnet matrix.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Nucleic Acid Isolation |Nucleic Acid Extraction |Cell culture |Proteomics |Purification and Detection |Protein Purification |DNA Purification |RNA Purification |Plant Tissue Processing |Tissue Analysis |Soil Analysis |Yeast Culture |Fungal Tissue Analysis |Microbiome Studies |Metabolite Extraction |Tissue Homogenization |Isolate Intact Organelles
Specifications
| SKU | 116915100 |
| Alternate Names | F Matrix |
| Application Notes | Lysing Matrix F is effective at breaking tough, hard, or brittle cell membranes. This is useful in applications such as isolation of intact organelles, and super-molecular structures where lysis conditions necessitate high impaction but low shearing. Matrix F is effective for applications such as RNA and protein isolation for samples in which Matrix A is too agressive. The irregular, angular shape of the Al2O3 and SiC crystals give lower shearing than the sharp edges of the garnet matrix. |
| Average Size | Aluminum Oxide: 1.6 mm |Silicon Carbide: 1.2 - 2.4 mm |
| Base Catalog Number | 6915050 |
| Bead Size | 1.6 mm |
| Capacity | 2 mL |
| FastPrep Instruments Models | ConeFlex Legacy (allows all FP24 adapters on FP96), CoolPrep (24x2 mL), HiPrep (48x2 mL), QuickFlex (96x2 mL), QuickPrep (24x2 mL), QuickPrep All Metal (24x2 mL) |
| Format | 2 mL tube |
| Hardness | Aluminum Oxide: Knoop 2000, Mohs 9 |Silicon Carbide: Knoop 2500, Mohs 9+ |
| Molecular Formula | Aluminum Oxide, Al2O3 |Silicon Carbide, SiC |
| Personal Protective Equipment | Personal-protective-equipment: If working with biological, toxic, hazardous or infectious samples; Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice. |
| Sample Type | Bacteria (Gram +/-), Animal Tissues, Fungi, Plants, Coral Emulsions, Mold |
| Specific Gravity | Aluminum Oxide: 3.95 |Silicon Carbide: 3.20 |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
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文献和实验相关专题 成功走出第一步：DNA提取 碱裂解法 大量提取质粒(500mL菌液) 1、取培养至对数生长后期(一般培养12～16小时)的含待提质粒的细菌 培养液于离心杯中，配平，4℃下4000rpm离心15min，弃上清，然后将离心管倒置于干净的约纸上使上清液全部流尽。 2、向盛有细菌 沉淀物的离心管中加入20mL冰冷的溶液Ⅰ，重悬沉淀(先加5mL溶液Ⅰ，用干净的玻璃棒搅拌均匀后，再加剩下的部分)。
的核酸；如果我们的目的是蛋白，那要根据蛋白的位置、特性等因素考虑裂解液，在提取蛋白后，再根据实验需要复性蛋白等。以下是细胞裂解中常用试剂和其作用：50 mM Tris-HCl pH 7.4（缓冲体系），150 mM NaCl（等渗体系），1 mM PMSF （强大的蛋白酶抑制剂），1 mM EDTA（变性剂和稳定剂），5 µg/ml Aprotinin（蛋白酶抑制剂），5 µg/ml Leupeptin（蛋白酶抑制剂），1% Triton x-100（破坏细胞），1% Sodium
质粒是存在于细菌染色体外的一个或多个能独立复制并稳定遗传的小型环状双链DNA分子，其分子量一般在0. 2～10kD 范围内。由于质粒分子小，便于分离和提取，可以携带目的基因进入细菌、动物细胞或植物体内进行扩增与表达。基因克隆实验中常将经改造后的质粒作为运送基因的载体，质粒D2NA提取效率及质量对后续实验步骤(如酶切、连接、转化大肠杆菌与PCR扩增等)的成功与否有着直接的关系，因而高效快速地从细菌细胞中分离纯化质粒具有重要意义[ 1 ] 。目前从细菌中提取质粒DNA多采用碱裂解法，该法操作简便
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