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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量现货
- 供应商:
广州威佳科技有限公司
- 现货状态:
现货
- 保修期:
请咨询
- 规格:
5*500 µl
概述
2.5 ml (5 x 500 µl), mixture of 10 recombinant proteins (10–250 kD), 8 blue-stained bands, and 2 pink reference bands (25 and 75 kD), 250 applications描述
Use improved Precision Plus Protein Dual Color Standards for molecular weight estimation on SDS-PAGE gels and western blots.- Mixture of 10 recombinant proteins of precise molecular weights (10–250 kD)
- 8 blue-stained bands and 2 pink reference bands (25 and 75 kD)
Features and Benefits
Increased overall brightness provides visual robustness throughout the entire protein electrophoresis and western blotting workflow. Improved Dual Color Standards are brighter than the competition on both gels and blots.Increased brightness makes it easier to:
- Identify target proteins on the gel or blot
- Monitor gel electrophoresis
- Confirm transfer quality
- Confidence after western blot processing
- Molecular weight confirmation
Packaging Options
| Number of Applications | Total Volume | Catalog Number |
| 10 | 50 µl | 1610374S |
| 50 | 500 µl | 1610374 |
| 500 | 2.5 ml (5 x 500 µl) | 1610394 |
Related Products
More Precision Plus Protein Standards:- Precision Plus Protein Unstained Standards
- Precision Plus Protein Prestained Standards
- Precision Plus Protein™ WesternC™ Standards
- Precision Plus Protein Unstained Standard Plugs, Strep-tagged, recombinant, pkg of 24 (1610378)
Related Categories
- Protein Ladders and Standards
- Precast Protein Gels
- Protein Electrophoresis and Blotting
- Western Blotting
Supporting Documents
- Precision Plus Protein Dual Color Standards Instruction Manual
- Strep-Tag Technology for Molecular Weight (MW) Determination on Blots Using Precision Plus Protein Standards Application Note
- The Little Book of Standards Reference Guide
Online Resources
- Protein Electrophoresis
- Western Blotting
技术指标
MW size range, kD10–250
染料类型
Dual Color
Volume, µl
2,500
Total number of applications
250
Loading buffer
30% (w/v) glycerol, 2% SDS, 62.5 mM Tris, pH 6.8, 50 mM DTT, 5 mM EDTA, 0.02% NaN3
保质期
Store for 6 months at 4°C or –20°C until expiration date
Recommended loading volumes*
Electrophoresis: 10 µl
Blotting: 7 µl
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文献和实验-PAGE,冰上电泳。用预染marker观察100kd的条带跑到胶的正中间位置时,才开始转膜,否则转膜效率降低) WB用膜类型、孔径: NC膜, 0.45 转膜方式(恒压、恒流):恒压,100V 转膜时间: 2 hours 转膜设备: BIO-rad 转膜设备。其间,准备一个冰盒,将电泳槽半埋到冰里。 转膜液的配制:(如甲醇的比例和是否加SDS以及多大质量浓度等): 2.9 g甘氨酸,5.8 g tris,0.37 g SDS,200 ml甲醇。
BSA 蛋白标准液、BCA 试剂、PBS。 1) 标准曲线:用 BSA 和 PBS 混合总体积为 20 μL 的溶液做标准曲线,具体操作如下:按照 0、0.2、0.4、0.6、0.8、1.0 含量稀释 BSA 蛋白标准品,每个浓度(0、4 μL、8 μL、12 μL、16 μL、20 μLBSA,其余用 PBS 补齐至 20 μL)梯度做一个复孔。 2) 样品稀释测蛋白浓度:10 倍稀释法:取 18 μLPBS+2 μL 样品上清(经上步处理的样品 12000 r/min 离心 5 min
测定是一种简单、精确的蛋白浓度定量方法。现成的1倍浓度染料和7 个预稀释浓度(0.125、0.25、0.5、 0.75、1.0、1.5、2.0 mg/ml)的蛋白标准品,让你拥有现成的检测工具。无需稀释标准品和染料,一步完成蛋白浓度定量。 Bio–Rad 蛋白测定 也是一种简单的蛋白浓度测定方法。该方法适应标准浓度测定、低浓度微量测定,或96孔微孔板的快速测定。它的基本原理和流程和上面的Quick Start Bradford都是一样的,不过要麻烦一点点。因为除了要稀释蛋白标准品,配成几个
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