赛默飞世尔Thermo Fisher 2-8C 30 HR

Northern Blotting方法(Howell Lab)

for at least 30 min before quantitation. Pipet up and down several times before taking 2 µl into 998 µl water. Read at 260 nm. RNA concentration (µg/µl) = OD x 500 x 0.04 µg/µl = OD x 20 Formaldehyde gel Boil 2 g agarose in 144 ml

Intracellular Cytokine Staining Protocol

.6 IFN-g PBMC PMA (30-50ng/ml)/Iono (1ug/ml) 5hr - Monensin 4S.B3 TNF-a PBMC PMA (30-50ng/ml)/Iono (1ug/ml

小RNA的Northern BLOT

#______________  1 μl T4 Polynucleotide Kinase, 10 U/μl Lot#______________  X μl Nuclease free H2O to 10 μl  37℃ at least 1 hr  After incubation, bring reaction volume up to 50 μl before cleaning up with spin column