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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70114-841-22
中文名称:
英文名称:INJECTION PLUG 50/PK
货期:热销品现货,其他请咨询




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文献和实验BAC DNA Purification For Injection
Equilibrate tip-500 column with 10 ml QBT buffer (750 mM NaCl, 50mM MOPS pH 7.0, 15% isopropanol, 0.15% Triton X-100) Apply filtered DNA solution to the column Wash column twice with 30 ml QC buffer (1.0 M NaCl, 50 mM MOPS pH 7.0, 15% isopropanol
for a few (about 6) filters. Heat seal bag and allow to warm in 65℃ oven for 15 minutes. Denature the radiolabelled DNA probe (1-2 x 106 cpm/filter) at 95℃, 5-10 minutes. Add probe at corner of sealed bag using a 25G needle and syringe. Heat seal below the injection site
Procedures for In Vitro Production of Bovine Embryos
with OMM + supplement microdrops (pre-equilibrated) Bench paper to cover surface 25 ml pipet automatic pipettor Mesh or styrofoam on counter X-plate Integrid petri-dish100 μm Falcon Cell Strainer50-ml Centrifuge Tubes, self standing with plug seal cap
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