赛默飞世尔Thermo Fisher COMB 40 SAM

SDS-PAGE

. However, they give innacurate molecular weights. 9. Samples of E. coli extracts: resuspend the pellet from 1 OD ml of culture in 50 µl sample buffer (1 OD ml= 1 ml of culture at OD600=1). On a BRL gel box with the 0.8 mm thick 20 well comb, load 20 µl of this (this is about 80

小RNA的Northern BLOT

cliPS . 10% Denaturing Acrylamide gel, 100ml (for 20x40 cm glass plates 1.5mm thick)  Swirl and warm up to 42C to allow for rapid polymerization (Heating is optional and not suggested for first-timers ) Pour gel:  Add 678 μl

TRICINE GELS Protocol (For Low MW proteins (

are very active in SDS sample buffer and can cause severe degradation. Once heated, sample could sit at RT for a short time until loading, or at -20°C for a long time. For a gel thickness of 0.75mm and 15 wells applied 10-25ug protein of a complex mixture