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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70311863433
中文名称:
英文名称:COMB 20 SAMPLE MC, 0.75MM THICK 0.75mmthick for Sub-gel maxi
货期:热销品现货,其他请咨询




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文献和实验(still on ice), and load immediately. For a gel thickness of 0.75mm and 15 wells applied 0.5 to 5ug for Coomasie Blue stain and 10 to 100-fold less protein for silver stain. If you do not know the electrophoretic pattern of your protein, load same sample
. However, they give innacurate molecular weights. 9. Samples of E. coli extracts: resuspend the pellet from 1 OD ml of culture in 50 µl sample buffer (1 OD ml= 1 ml of culture at OD600=1). On a BRL gel box with the 0.8 mm thick 20 well comb, load 20 µl of this (this is about 80
cliPS . 10% Denaturing Acrylamide gel, 100ml (for 20x40 cm glass plates 1.5mm thick) Swirl and warm up to 42C to allow for rapid polymerization (Heating is optional and not suggested for first-timers ) Pour gel: Add 678 μl
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