赛默飞世尔Thermo Fisher PLUG CAP 16

Procedures for In Vitro Production of Bovine Embryos

with OMM + supplement microdrops (pre-equilibrated) Bench paper to cover surface 25 ml pipet automatic pipettor Mesh or styrofoam on counter X-plate Integrid petri-dish100 μm Falcon Cell Strainer50-ml Centrifuge Tubes, self standing with plug seal cap

Northern Blotting方法(Howell Lab)

Briefly spin up/down in the microfuge. Following the protocol from the Amersham Multiprime Kit (RPN.1601): Add 10 µl of Buffer (solution 1, blue cap vial) Add 5 µl of Primer (solution 2, black cap vial) Add 5 µl of [a-32P]dCTP (3000

小RNA的Northern BLOT

#______________  1 μl T4 Polynucleotide Kinase, 10 U/μl Lot#______________  X μl Nuclease free H2O to 10 μl  37℃ at least 1 hr  After incubation, bring reaction volume up to 50 μl before cleaning up with spin column