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999
- 供应商：
北京泽平
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- 保修期：
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- 规格：
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货号：T_701855-16
中文名称：
英文名称：BARIUM CHLORIDE, 20%, 500 ML
货期：热销品现货，其他请咨询
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文献和实验Purification of Plasmid from 50 ml-culture
is visible at the bottom of the tube, vortex well to dissolve the pellet. Incubate the tube at 37 C for further 30 min. 16. Add 240 ul of 2M NaCl, 20% PEG8000. (PEG6000 supplied from Japanese suppliers is essentially equivalent to PEG8000, and works
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
for the restriction enzyme to be used in Step 16). 13. Pellet the cells by centrifugation. Resuspend in 500 µL of 1.14X restriction enzyme buffer. Transfer to 1.5-mL tubes. 14. Add 7.5 µL of 20% SDS (to a final concentration
7b. Pool the supernatants from step 6 into 500 ml bottles and add DNase-free RNase A and RNase T1 such that the final concentration of RNase A is 40 ug/ml and RNase T1 is 40 U/ml. Incubate in a 37degC water bath for 30 minutes. 8b. Add an equal
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