赛默飞世尔Thermo Fisher X20 Rack fo

Nucleolar Isolation Protocol

that all the cells are detached. Prolong incubation if needed. Add into each dish 8 ml of pre-warmed medium, pipette up and down so that all the cells are collected as a single-cell suspension. Pool all the harvested cells into 2x 50ml Falcon tubes. For some strains

Procedures for In Vitro Production of Bovine Embryos

with OMM + supplement microdrops (pre-equilibrated) Bench paper to cover surface 25 ml pipet automatic pipettor Mesh or styrofoam on counter X-plate Integrid petri-dish100 μm Falcon Cell Strainer50-ml Centrifuge Tubes, self standing with plug seal cap

Cajal Body Isolation Protocol

the mixture into two portions, approx. 20ml each, in two 50ml Falcon tubes (Figure 2). 9. Centrifuge at 3000g (3500 rpm, Beckman GS-6 centrifuge, GH-3.8 rotor) for 10 min at 4°C.