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- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70311542143
中文名称：
英文名称：Sieve stainless steel 80µm
货期：热销品现货，其他请咨询
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文献和实验syringe with ice-cold PBS. 3. Fit a blunted 21 gauge needle (nip the bevel off with pliers). 4. Squirt about 3ml of the ice-cold PBS down the shaft of each excised femur. 5. Catch the tissue fragments in a hemispherical 80-mesh stainless steel sieve
stainless steel sieve perched on top of a plastic tube. 6. Rub the tissue fragments through the sieve using the bottom of another plastic tube, and rinse through with the remaining ice-cold PBS. 7. Centrifuge the sample. Isolation of bone marrow
Immunostaining of Paraffin Sections
Procedure: 1) Fix tissues for 3 hr on ice in 4 formaldehyde (2.5 ml of Polysciences #18814 made up to 10 ml in 80 mM NaPO4 [3.2 ml of 1 M NaPO4] pH 6.8 containing 0.2 gm of sucrose). Wash for 2 hr
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