赛默飞世尔Thermo Fisher SCREW CAP G

AFFINITY PURIFICATION OF ANTIBODIES

1. Affigel 102 (BIORAD) washed in PBS on a scintered glass funnel (3ml/column). 2. Transfer to 15ml screw cap tube in minimal volume. 3. Add 7mg SMCC (in DMF) to 2.5ml beads and mix for 2h @ RT. 4. Wash beads again in PBS. 5. Resuspend in minimal

常用缓冲液配制

in screw-cap vial, store at -20 C. 10 X REact buffer 3 : for, e.g. Eco RI (5.0 ml) 1 X Conc Stock 500 mM 1M Tris (pH 8.0) 2.5 ml 100 mM 1M MgCl2 0.5 ml 1 M 5 M NaCl 1.0 ml 25 mM Spermidine(3HCl) 0.032 g H2O 1.0 ml Mix, aliquote 2 ml

Saturation Analysis of Ligand Binding Using a Centrifugation Procedure

Sorvall RC‐5B refrigerated superspeed centrifuge and SH‐MT and SS‐34 rotors (DuPont), or equivalent 1.5‐ml O‐ring screw‐cap test tubes and screw‐cap microtube rack (Sarstedt