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- 2025年07月15日
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- 技术资料
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999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_7012665-16
中文名称：
英文名称：EDTA, 2.5% AQUEOUS, 1 L
货期：热销品现货，其他请咨询
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文献和实验Preparation of End-Labeled DNA Probes by Conventional Kinase for DNA Footprinting Analysis
1 μl of γ-[32 P]-ATP (CAUTION! see Hint #1) 1 μl of T4 Polynucleotide Kinase enzyme Total volume is 40 μl. 2. Incubate at 37°C for 25 min. 3. Add another 0.5 μl of T4 Polynucleotide Kinase enzyme
The ribonuclease protection assay (RPA)
P]UTP 1 µl T7 RNA polymerase (Keep at -20°C until use, return to -20°C immediately). Mix by gentle pipetting or flicking and quick spin in a microfuge. Incubate at 37°C for 1 hour. 2. Terminate the reaction by adding 2 µl of DNase. Mix
A quick RNA mini-prep for Neurospora mycelial cultures
15. Spin 10-20 min in microfuge. Wash twice with 70 EtOH and dry pellet. Resuspend in 200 µl of sterile RNase-free gd H2O. Store at -70°C for up to three months. Spectophotometric quantification may be done at this point. 16. Load 1 µl
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