赛默飞世尔Thermo Fisher POT HYDROXI

Use of a Novel 5′‐Regioselective Phosphitylating Reagent for One‐Pot Synthesis of Nucleoside 5′‐Triphosphates from Unprotected Nucleosides

Figure 1.30.1 General scheme for one‐pot synthesis of natural and sulfur‐ or selenium‐modified 5′‐triphosphates from unprotected nucleosides. Abbreviations: DMF, N , N ‐dimethylformamide; TBA, tributylamine

Two‐Step, One‐Pot Synthesis of Inosine, Guanosine, and 2′‐Deoxyguanosine O6‐Ethers via Intermediate O6‐(Benzotriazol‐1‐yl) Derivatives

Harwood, E.A., Sigurdsson, S.T., Edfeldt, N.B.F., Reid, B.R., and Hopkins, P.B. 1999. Chemical synthesis and preliminary structural characterization of a nitrous acid interstrand cross‐linked duplex DNA. J. Am. Chem. Soc. 121:5081‐5082

（共享）免疫组织化学染色FAQ

water. Cool. Adjust pH to 7.0 with 1 M potassium hydroxide (about 35 mL) Dilute to 100 mL with more water. Washing solution: 25 mL 1.0 M potassium citrate buffer 50 mL 0.1 M magnesium sulfate heptahydrate (F.W. 246.5) 50 mL 0.1 M N-ethyl