赛默飞世尔Thermo Fisher 三角烧瓶 FLASK

Replication timing by density transfer

. (I add 0.1 mg/ml ampicillin if I'm feeling paranoid.) The doubling time of RM14-3a is ~ 2.6 hr at 23o C in glucose and 6-8 hr (depending on aeration) in acetate--longer if you're selecting for a plasmid. RM14-3a is MATa, cdc7-1ts , bar1, trp1-289

Plant DNA methylation analysis by bisulfite genomic sequencing

at sites outside of region intended for amplification to resolve structures like direct or inverted repeats that could interfere with PCR (see comment 2). To a 1.5ml reaction tube, Add genomic DNA dissolved in bidist. water (e.g. 2.5µg

Preparation of Tonsil Lysate

Stainless steel mesh screen (200 mesh=74 mm,Type 316; Tylinter,Mentor,Ohio) RPMI-1640 medium Triton X-100 Lysis Buffer: 50 mM Tris-HCl,pH 7.5 150 mM NaCl 0.02% NaN3 10 mg/ml Aprotinin 10 mg/ml Pepstatin 10 mg/ml Leupeptin 1 mM PMSF (Made fresh