赛默飞世尔Thermo Fisher 三角烧瓶 FLASK

Replication timing by density transfer

4 , 2.0 M NaCl, pH 6.8 T10 E100 Add to 350 ml dH2 O: 18.61 g Na2 EDTA?H2 O 1.5 g NaOH pellets 5 ml 1M Tris-HCl pH 7.5 Stir until the solids have dissolved, bring the pH to 7.5 with concentrated NaOH (2 O

Plant DNA methylation analysis by bisulfite genomic sequencing

) at temperature appropriate for used restriction enzyme; Add 0.5ml Tris-buffer-saturated phenol-chroroform, vortex; Centrifuge with Eppendorf 5414 or equivalent at 10000 x g for 2 minutes; Transfer the upper aqueous phase to clean 1.5ml

Preparation of Tonsil Lysate

Stainless steel mesh screen (200 mesh=74 mm,Type 316; Tylinter,Mentor,Ohio) RPMI-1640 medium Triton X-100 Lysis Buffer: 50 mM Tris-HCl,pH 7.5 150 mM NaCl 0.02% NaN3 10 mg/ml Aprotinin 10 mg/ml Pepstatin 10 mg/ml Leupeptin 1 mM PMSF (Made fresh