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999
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北京泽平
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货号:T_70310399800
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英文名称:VOL. FLASK A AMB 1000 ML NS 24/29 Blaubrand 1000 ml
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文献和实验ES / MEF cell culture and electroporation of targeting construct
with 70% ethanol and transfer the contents of the vial into one 75 cm2 flask (T-75) containing 20 ml of MEF media. Place the MEFs in a 37o C, 5% CO2 , 86% humidity incubator. Every frozen MEF preparation thaws a little differently. If on Day 1, the MEFs
ES / MEF cell culture and electroporation of targeting construct
ml of MEF media. Place the MEFs in a 37oC, 5% CO2, 86% humidity incubator. Every frozen MEF preparation thaws a little differently. If on Day 1, the MEFs are only 50% cconfluent, thaw another vial into your ongoing T-75 MEF flask. It is important
Construction and Characterization of Adenovirus Vectors
as described above or in suspension. Grow the cells in suspension in 1X maintenance medium in suspension flasks, agitated at 70 rpm. When they reach a density of 5 x 105 cells/mL, dilute 1:2 or 1:3 with 1X maintenance medium. CsCl (cesium chloride
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