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文献和实验Plant DNA methylation analysis by bisulfite genomic sequencing
(see comment 3); Centrifuge Sigma 1-15K (or equivalent) >15 minutes at 4°C 12000rpm (12000 X g); Discard supernatant, add 1.0ml 70% ethanol (room temperature), vortex; Centrifuge Sigma 1-15K (or equivalent) >15 minutes at 4°C 12000rpm
Chromatin Immunoprecipitation (ChIPs) Cloning Protocol (Farnham Lab)
, it may also be helpful to add 0.1 grams of glass bead (212-300 microns; Sigma) prior to sonication). For cloning purposes, a size range of 1 kb to 3 kb is desirable. After sonication, microfuge samples at 14,000 rmp for 10 minutes at 4 0 C. At this point
Anderson, D. and Plewa, M.J. 1998. The international comet assay workshop. Mutagenesis. 13:67‐73. Böcker, W., Bauch, T., Müller, W.‐U., and Streffer, C. 1997
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