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- 库存:
999
- 供应商:
北京泽平
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EA
货号:T_7015807-16
中文名称:
英文名称:PHOSPHATE BUFF, PH 6.8, 500 ML
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文献和实验Calcium Phosphate Transfection of Neurons in Primary Culture
after transfection wash cells 3X with transfection media (serumfree, add, aspirate), then add fresh transfection media (don't let cells dry out) volume of transfection media: well of 24-well plate: 500ul
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
:chloroform:isoamyl alcohol, UltraPure (25:24:1 v/v/v; Invitrogen) Phosphate-buffered saline (PBS; GIBCO 20012) Protease inhibitor cocktail (Sigma P8340) Proteinase K (10 mg/mL in TE buffer, pH 8.0; Invitrogen) PureLink HQ 96 Mini
Native Chromatin Preparation and Illumina/Solexa Library Construction
off supernatant completely. 14. Resuspend the cells completely into appropriate volume of T cell isolation buffer. Use 3 mL for 250-300 million cells. 15. Proceed to magnetic separation using an LS column (Step 16).
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