赛默飞世尔Thermo Fisher INDICATOR L

ChIP Protocol（Mirmira Lab）

Reagents: ChIP sonication Buffer (1% Triton X-100,0.1% Deoxycholate,50 mM Tris 8.1,150 mM NaCl,5 mM EDTA):10 ml 10% Triton X-1001 ml 10% Deoxycholate5 ml 1 M Tris-Cl pH 8.11 ml 0.5 M EDTA3 ml 5 M NaCl80 ml WaterJust before use,add 10μl Aprotinin,10μ

Northern Blotting方法(Howell Lab)

buffer. Pour GITC/Cell mix into (14x95 mm) polyallomer tubes with 4 ml CsCl buffer. Balance and top off tubes with more GITC buffer. Spin tubes on the SW40Ti rotor for 20 hr at 32,000 rpm (18℃). Pour off liquid and keep tubes

Chromatin Immunoprecipitation (ChIPs) Protocol for Tissues(Farnham Lab)

buffer       5 mM PIPES pH 8.0       85 mM KCL       0.5% NP40       protease inhibitors  Nuclei Lysis buffer       50 mM Tris-Cl pH 8.1       10 mM EDTA       1% SDS       protease inhibitors IP Dilution buffer       0.01% SDS       1.1% Trition X 100