赛默飞世尔Thermo Fisher INDICATOR H

Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos

as stage X, nearly 24 h earlier in development than most electroporation protocols. Compared to in ovo electroporation, the ex ovo method allows easier embryo orientation (the posterior half of the embryo is darker than the anterior half

Use of Spectral Fluorescence Resonance Energy Transfer to Detect Nitric Oxide‐Based Signaling Events in Isolated Perfused Lung

   Karpova, T.S., Baumann, C.T., He, L., Wu, X., Grammer, A., Lipsky, P., Hager, G.L., and McNally, J.G. 2003. Fluorescence resonance energy transfer from cyan to yellow fluorescent protein detected by acceptor

Stripping Western Blots

. Add 1ml lysis bufferb and vortex briefly. 11) Repeat Step #10 2-3 more times. 12) After last wash, aspirate supernatant and remove remainder of wash buffer with fine gauge (>22g) needle. Process for kinase reaction (as indicated) or add 40-80μl 1X