Sartorius赛多利斯13430-100------KG

体外荧光法检测核内体早期动力学

. Remove the supernatant and resuspend the hexokinase pellet in 5 μl glucose solution (250 mM). Cytosol mix (volumes correspond to one reaction of 50 μl; scale up accordingly): 100 μg cytosol (usually 10�17 μl, depending

Cell Culture Media and Solutions

the tubes at -80 degrees C for up to one year. 700 ml RPMI-1640 with 2mM L-Glutamine 200 ml fetal bovine serum (FBS) 100 ml dimethyl sulfoxide (DMSO, Sigma) -------- 1000 ml total volume Note: Filter sterilize media and FBS

Random Primer DNA labeling

-----------  = dpm/µg (ng DNA synth. + ng input DNA) x 0.001µg/ng Note The factors 20 and 50 are derived while using 5µl of 1:100 dilution for DE-81 filter-binding assay