赛默飞世尔Thermo Fisher FLUORIDE ST

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

for the restriction enzyme to be used in Step 16). 13. Pellet the cells by centrifugation. Resuspend in 500 µL of 1.14X restriction enzyme buffer. Transfer to 1.5-mL tubes. 14. Add 7.5 µL of 20% SDS (to a final concentration

Convenient and Efficient Approach to the Permanent or Reversible Conjugation of RNA and DNA Sequences with Functional Groups

hydrogen carbonate (NaHCO 3 ; Fisher) Anhydrous sodium sulfate (Fisher) Ammonium fluoride (Aldrich

Lacrimal Experimental Protocol (4/4/94)

enzyme solution mixing together 500 µl of hyaluronidase (Worthington 'HSEP'; stock 6980 U/ml), 500 µl of collagenase (Worthington; 1:1 mixture of types II and IV; stock 4000 U/ml), and 100 µl of DNase (stock 1000 U/ml) to 8.4 ml of DMEM/gent.