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999
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北京泽平
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热销品大量现货,其余请咨询
- 保修期:
1年
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EA
货号:T_70103-344-67
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英文名称:INSERT .1ML GLS LV W/SP 100/CS
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文献和实验Expression Library Screening (Procaryotic) Using AP-Fusion Proteins
) 20% (w/v) Maltose (100 x) LB media LB tetracycline plates or NZY tetracycline plates 12.5 g/l Tetracycline Stock (1000 x) XL1-Blue MRF' strain (general use)
can be done overnight at RT with constant agitation. 3) Washed stripped membrane at least 3x for 10min each with PBST, then re-block (in PBST+NFDM or PBST+BSA) overnight at 4oC. Stripping Buffer: 62.5mM Tris pH 6.8 2% SDS 100mM b-ME Quick Mix
(i.e. if using 100 mls of Urea/Acrylamide) 10% APS (Date:________) and 67.8 μl TEMED (Lot#___________), swirl to mix and start pouring immediately with a 50 ml pipette. Hold the plates at approximately 45 degrees
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