赛默飞世尔Thermo Fisher HYDROCHLORI

小RNA的Northern BLOT

#______________  1 μl T4 Polynucleotide Kinase, 10 U/μl Lot#______________  X μl Nuclease free H2O to 10 μl  37℃ at least 1 hr  After incubation, bring reaction volume up to 50 μl before cleaning up with spin column

Imaging Protein‐Protein Interactions by Förster Resonance Energy Transfer (FRET) Microscopy in Live Cells

of fluorescence resonance energy transfer using fluorescence microscopes. Biophys. J. 81:2395‐2402.    Xu, X., Meier‐Schellersheim, M., Jiao, X., Nelson, L.E., and Jin, T. 2005

DNA Solutions （DNA分离）

xylene cyanole FF (Kodak T-1579) ddH2 O to 10 ml (store at -20deg.C). Alkaline lysis solution (NaOH/SDS) : 0.2 N NaOH, 1% SDS in ddwater. 20 ml of 1 N NaOH (or 0.8 gms) 10 ml of 10% SDS (or 1.0 gms) ddH2 O to 100 ml (make fresh) 15% Ammonium