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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70105-403-00
中文名称：PCR冰盒
英文名称：EPPENDORF PCR TUBE COOLER BLUE
货期：热销品现货，其他请咨询
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文献和实验Inverse PCR & Cycle Sequencing
I. Quick Fly Genomic DNA Prep Standard fly mini prep (30 flies) resuspended in 150 ul TE 1) Collect 30 anesthetized flies in eppendorf tube and freeze at -80°. 2) Grind flies in 200 µl Buffer
Reverse Ligation Mediated RT - PCR
into each of four tubes (ie an 'A' tube, a 'C' tube etc.) Sequencing mix: 8µl H2 O 5µl 5x sequencing reaction buffer 2µl 1µM labelled primer 1µl of the diluted PCR1reaction 5U Promega sequencing grade
Inverse PCR Cycle Sequencing of P Element Insertions for STS Generation
I. Quick Fly Genomic DNA Prep Standard fly mini prep (30 flies) resuspended in 150 ul TE 1) Collect 30 anesthetized flies in eppendorf tube and freeze at -80°. 2) Grind flies in 200 µl Buffer A with disposable tissue
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