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999
- 供应商:
北京泽平
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热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_701AA1003418
中文名称:
英文名称:MOLY RD 12.7MM DIA 99.95% 50G
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文献和实验A safe method of extracting DNA from Coccidioides immitis
in the saprophytic phase at 37 C in 100 ml of 2X GYE medium (20 g glucose and 10 g yeast extract in 1 liter medium, autoclaved for 15 min.) in 500 ml plastic Nalgene flasks with plastic foam stoppers (Identi-plug, Fisher). When there has been sufficient growth
A safe method of extracting DNA from Coccidioides immitis
(Identi-plug, Fisher). When there has been sufficient growth, the whole container is transferred to an autoclave and steamed at 1 atm (100 C) for 15 min. This raises the temperature of the culture to about 95 C (as measured in trials with a thermocouple
of fixative.): Lidocaine 50 mg per mL, dissolved 95% EtOH (warm to dissolve). Add slowly to perfusion solution with stirring to make final lidocaine solution concentration wanted (e.g. 1mg/mL = 0.1%) Note: Do not add the lidocaine directly
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