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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70311360604
中文名称:
英文名称:X100 FILTER GLASS GF/F 42.5MM
货期:热销品现货,其他请咨询




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文献和实验Protocol for Trichloroacetic Acid (TCA) Precipitation
sperm DNA, Ambion Cat # 9680 ; dilute to 1 mg/ml in water) Glass fiber filters to catch precipitate (e.g., Whatman GF/C filter circle; or Schleicher & Schuell #34 2.7-cm glass filter circles, Cat #10373828) Aqueous scintillation fluid
Tris(PH=6.8) 10% SDS 40 µl 10% ammonium persulfate 40 µl TEMED 7 µl C. Preparation of gel 1. Assemble the glass plates and spacers (0.75 mm thick). 2. Pour the running gel to about 1 cm below the wells of the comb (~3.2 ml). 3. Seal with 1 ml
in the stir bar. 13.Bring the vlume up t 1 liter by adding mre f the Lysis buffer. 14.Add Tritn-X100 t a final cncentratin f 2% by vlume. Nte: The Tritn-X-100 will nt disslve immediately.It will g int slutin slwly ver night. 15.Stir gently ver night
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