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英文名称:DE TUBE SULFUR DIOXIDE 10/PK
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文献和实验Single and Double FISH protocols for Drosophila
to the bottom of the tube (1-3hr or overnight at 4oC) before resuspending and mounting. 10. Transfer the embryos to a clean slide in ~ 80 ul mountant and cover with a 25 X 50 mm coverslip. Seal the edges with nail polish. Slides can be stored for weeks at 4oC
., ) Dry N ,N ‐diisopropylethylamine (DIPEA; Aldrich) Methylene chloride (CH 2 Cl 2 ; Fisher) and anhydrous methylene chloride (Acros
8. 3M sodium acetate. 9. Cold 100% ethanol. 10. Cold 70% ethanol. 2.2 Embryo Collection and Fixation 1. Chlorine bleach solution diluted 1:1 with water. 2. 20 mL glass scintillation vials (Fisher Scientific
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