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999
- 供应商:
北京泽平
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货号:T_70310171773
中文名称:
英文名称:X96 TUBE 2D BARCODED STERILE SCREWTOP 4 RACKS OF 24 tubes 12mL (pack of 96)
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文献和实验ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
V 4. ASAP using a pasteur pipette transfer cells from curvette A to a 10 ml tube containing 4 mls medium, then plate 5 x 1 ml cells onto five 10cm dia petri dishes containing NeoPMEF's and 10 ml medium. Label plates 4-8. B to a 10 ml tube containing 1ml
Procedures for In Vitro Production of Bovine Embryos
tubes (05-539-5)18 g 1 ½” needles (14-826-5D)50 ml sterile centrifuge tubes (05-538-67) 60 X 15 petri dishes (08757100B) (Falcon 351007) Absorbent paper (14-206-65)Beakers 10 ml (02-540C) 50 ml (02-540G) 400 ml (02-555
ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
a multichannel pipette disperse cells as before, transfer 25 ul into 96 well plate containing 10% DMSO in medium. Then using a Gilson and a sterile tip transfer remaining cells to a 24 well plate with 1 ml medium with LIF (no feeders) in each well, 24 wells
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