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货号：T_70108-801-244
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英文名称：S/P2 DIALYSIS TRIAL 12-14K45MM
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文献和实验Chromatin Immunoprecipitation (ChIPs) Protocol (Farnham Lab)
Biggin, Dave Allis and Richard Treisman plus a fair amount of trial and error. We have successfully used this protocol with NIH 3T3, Friend, HeLa, Raji and CHO cells. However, you may have to optimize conditions for your specific cell type.
Chromatin Immunoprecipitation (ChIPs) Protocol for Tissues(Farnham Lab)
on the rotating platform at room temp for 15 minutes, no longer. 13. Microfuge samples. Save the supernatant from the "no antibody" sample as "total input chromatin". 14. Wash pellets twice with 1.4 mls of 1X dialysis buffer (**if you are using
Chromatin Immunoprecipitation (ChIPs) Protocol for Tissues(Farnham Lab)
temp for 15 minutes, no longer.13. Microfuge samples. Save the supernatant from the "no antibody" sample as "total input chromatin".14. Wash pellets twice with 1.4 mls of 1X dialysis buffer (**if you are using a monoclonal antibody, omit the sarkosyl
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