赛默飞世尔Thermo Fisher POT CHLORID

Two‐Step, One‐Pot Synthesis of Inosine, Guanosine, and 2′‐Deoxyguanosine O6‐Ethers via Intermediate O6‐(Benzotriazol‐1‐yl) Derivatives

Imidazole, 99% pure (Sigma) tert ‐Butyldimethylsilyl chloride (TBDMS‐Cl), 98% pure (Acros) Hexanes, ACS grade (Fisher

DNA Solutions （DNA分离）

. 2 ml 1 M KCl 1 ml 1 M Tris-HCl, pH 7.6 1 ml 1 M MgCl2 0.1 ml 1 M DTT ddH2 O to 10 ml RNase T1 : 100 U/ul in 50 mM Tris-HCl, pH 7.6 100 μl RNase T1 (Sigma R-8251) (100,000 U/0.2 ml) 25 μl 1 M Tris-HCl, pH 7.6 375 μl ddH2 O 500 μl 10

TISSUE CULTURE ON COVERSLIPS

Glacial Acetic Acid, prepared fresh. Hypotonic solution: 0.8%NaCitrate/0.075 M KCl: 1.6g NaCitrate, 1.68g Potassium Chloride dissolved in 500ml dH2 O. 1X Trypsin EDTA solution: 10ml stock trypsin solution (10X trypsin in saline), 0.1g