sartorius赛多利斯12602--50----ALNM

Optical Filters for FISH

Fluorescence in situ hybridization (FISH) is strongly dependent on good hardware, i.e., the microscope and the filter sets used. Here an introduction to fluorescence, fluorescence spectra and fluorescent dyes, as well as filters

Overgo Probing of High-Density Filters

1.50 ml of warmed hyb solution is added to a 30 cm x 4 cm hyb bottle. Filters are prewet with warmed 2xSSC and then each filter is rolled and inserted into the bottle. With the cap on, the bottle is rotated to allow the filter to unroll slowly

Screening BAC filters with non-radioactive probes

-hybridization and 5 ml for probe solution.)   Pre-hybridization of filters: more than 30 min at 42C After pre-hybridization, transfer the hybridization solution to 50 ml tube, add labeled probe (48 ml), mix