Sartorius赛多利斯13400--80------NG

Cell Culture Media and Solutions

the tubes at -80 degrees C for up to one year. 700 ml RPMI-1640 with 2mM L-Glutamine 200 ml fetal bovine serum (FBS) 100 ml dimethyl sulfoxide (DMSO, Sigma) -------- 1000 ml total volume Note: Filter sterilize media and FBS

Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence and VE-DIC

this is using a glucose oxidase/glucose/catalase mix (OS mix). The component enzymes are stored as 100X stocks at -80¡C and thawed and used for ~2 hours after mixing. It is important to keep the OS mix in a sealed tube on ice. The principle by which the mix

Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence and VE-DIC

for several hours; spin at 70K in TLA100.4 and collect clear middle layer. Measure protein concentration by Bradford using BSA as a standard and dilute to 5 mg/ml. This can be filtered if desired then aliquoted and stored at -20¡C or -80¡C) 10 mg/ml HSA or high