Sartorius赛多利斯17576----------QM

Single Primer ("Semi-Random") P

a biased ratio of Po and Pn. Make 3 reactions with varying [Mg++]: 15 µL H2O 3 µL 10x H, M or L-PCRB 3 µL 4 dNTPs @ 2 mM each 3 µL Po @ 5 µM 3 µL template diluted to about 1 ng/µL 3 µL "T/TS" @ 0.4 u Taq/µL -------- 30 µL

Preparation of Carboxypeptidase Y and Its Properties

is immersed in a water bath at 30℃ during kneading of the mixture. After liquefaction, add 600ml of distilled water, the mixture is stirred. The pH of the mixture is adjusted to 7.0 with 1 M NaOH under vigorous stirring, and the whole is kept at 25

Isolation of cell nuclei for the application in the cell-free system

7.4) 10 mM KCl 2 mM MgCl2 1 mM DTT 0.1 mM PMSF 2 µg/ml Leupeptin 2 µg/ml Pepstatin 2 µg/ml Aprotinin 10 ml of 50 mM PIPES, pH 7.4 500 µl of 1 M KCl 1.0 ml of 100 mM MgCl 50 µl of 1 M DTT 500 µl of 10 mM PMSF 100 µl of 1 mg/ml