Sartorius赛多利斯12303-100------GC

DGK Membrane Preparation

5 mM b-mercaptoethanol 390 µl TNE buffer for 1 L 25 mM Tris 3.03 g 100 mM NaCl 5.84 g 1 mM Na2 EDTA (pH 8.0) 0.37 g 5 mM b-mercaptoethanol 390 µl TEM buffer for 1 L 25 mM Tris 3.03 g 1 mM Na2 EDTA (pH 8.0) 0.37 g 5 mM b

Preparation of Brain Membrane Fractions

in approximately 5 ml water. Add sucrose and water to give a final volume of 45 ml 1.2 M sucrose, add HEPES to 5 mM and the protease inhibitors as above. Homogenize in glass FEP polymer-homogenizer (3 strokes, 1000 rpm).Spin at 9200 x g for 20 min.Resuspend pellets

Protocol for isolating DNA from embryo yolk sacs, or toes, or 2 mm tail for PCR

(not Tris base). The pH is about 5. Don't adjust the pH. For yolk sac/placenta/tail/toes 2. Add 40 µl (100 µl for tail/toe) 25 mM NaOH / 0.2 mM EDTA. 3. Place at 95°C for 20 minutes (in a thermal cycler) 4. Add 40 µl (100 µl for tail/toe) 40 mM Tris