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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
Mouse IgG1
- 保存条件:
2 - 8℃避光,切勿冻存
- 克隆性:
UP-H2
- 标记物:
PE
- 级别:
流式抗体
- 库存:
现货
- 供应商:
上海经科化学科技有限公司
- 应用范围:
流式检测
- 浓度:
5 μL /Test
- 靶点:
CD300E
- 规格:
100T(F11300E02-100)/25T(F11300E02-25)
| 规格: | 100T(F11300E02-100) | 产品价格: | ¥2072.0 |
|---|---|---|---|
| 规格: | 25T(F11300E02-25) | 产品价格: | ¥935.2 |
| 商品名 | Anti-Human CD300E, PE (Clone: UP-H2)检测试剂 |
|---|---|
| 靶点 | CD300E |
| 标记 | PE |
| 仪器 | 适用488nm, 532nm, 561nm激光器的流式细胞仪 |
| 亚型 | Mouse IgG1 |
| 克隆号 | UP-H2 |
| 推荐应用 | 流式检测 |
| 用量 | 5 μL /Test |
| 纯化 | 亲和层析 |
| 储存液 | 含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2) |
| 保存条件 | 2 - 8℃避光,切勿冻存 |
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文献和实验分离人单核细胞和中性粒细胞-Purification of human mononuclear cells and neutrophi
Histopaque 1077 18 ml cold H2 O 2 ml 10x PBS M199 for HUVECs: 1L powder pocket M199 + 35g NaHCO3 + 25mM Hepes + 5 mM glutamine + 50 ug/ml Gentamycin. 1M Hepes: 119.1 g Hepes + 500 ml dH2 O. Media A: 1X HBSS (10X:50ml) + 10
Native Chromatin Preparation and Illumina/Solexa Library Construction
carried over through protein-protein interactions. The protocol includes purification of human CD4+ T cells from lymphocytes and chromatin fragmentation using micrococcal nuclease (MNase) digestion, followed by chromatin immunoprecipitation (ChIP
Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD
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