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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海经科化学科技有限公司
- 抗体英文名:
KLH | Keyhole limpet hemocyanin
- 规格:
50ug
| Immunogen |
| Purified keyhole limpet hemocyanin (KLH), whole molecule, |
|
Host Rabbit
|
|
Clonality Polyclonal
|
|
Purity Affinity purified serum in PBS, pH 7.4
|
|
Format Lyophilized
|
|
Quantity 50 µg
|
|
Reconstitution For reconstitution add 50 µl of sterile water.
|
|
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
|
|
Tested applications ELISA (ELISA), Immunolocalization (IL), Western blot (WB)
|
|
Recommended dilution
|
| 1: 10 000 (ELISA), 1: 10 000 (WB), 1: 1000 (IL) |
|
Expected | apparent MW
|
| ca. 400 kDa/subunit |
名称:KLH | Keyhole limpet hemocyanin
货号:AS99 001
品牌:Agrisera
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文献和实验The T-Dependent Antibody Response to Keyhole Limpet Hemocyanin in Rodents
production) to an introduced antigen (i.e., keyhole limpet hemocyanin (KLH)) in rats or mice treated with a chemical (e.g., a known immunotoxicant and/or a new drug candidate). The TDAR model of competent immune function requires the participation of multiple
Preparation Of Peptide-KLH Conjugates For Immunization
will not couple well. You may need to regenerate the thiol groups by reducing the peptide with excess DTT and running a P2 column.2. Coupling of Peptide to KLHThis recipe is for two bunnies for about five injections per bunny(1) Weigh out 100mg of keyhole limpet
How Do I make an Antibody against a Peptides
-Activated Carrier Proteins (Figure 1). If your hapten does not have a sulfhydryl group, you can use EDC chemistry (Figure 2) to couple amines (lysine) or carboxylic acids (glutamic, aspartic acid or 5'-phosphate) to the carrier protein. Keyhole limpet
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