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- Biorigin
- BN25340-467g
- 2025年12月22日
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文献和实验SOFT AGAR ASSAY FOR COLONY FORMATION
Note: All volumes are calculated to cater for four plates per point. Base Agar 1. Melt 1% Agar (DNA grade) in microwave, cool to 40 in a waterbath. Warm 2X RPMI + 20% FCS to 40 in waterbath. Allow at least 30 minutes for temperature
Hard Agar Medium for Egg Collection Plates
This is a firm medium that keeps well and makes a good base for egg collecting. Wrap filled petri dishes and store at 4-- 14o C (they should keep for at least 6 weeks). Substitute molasses for the corn syrup if you prefer
Materials (Solutions are all available from the media room) 200ml bottle of 2x SD 200ml bottle of 4% agar -- make sure to sign it out 40% glucose CSM minus the relevant marker(s), as powder Stack
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