Sartorius赛多利斯11106-293------GC

Construction and Characterization of Adenovirus Vectors

stock solution with H2 O and sterilize by autoclaving for 45 min at 121°C. Complete medium Dialysis buffer (10 mM Tris-HCl, pH 8.0) Sterilize by filtration through a 0.2-µm filter. DNase I (Ad) Ethidium bromide

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

   3.75ml 4M NaCl       3ml 10% Triton X (Tx-100)       300µl 10% NAD       21.45ml H20 ChIP wash buffer       30ml final volume:       150µl 2M Tris       1.7ml 4.4M LiCl       60µl 0.5M EDTA       1.5ml 10% NAD       1.5ml 10% NP-40       25.09ml

基于PCR技术的染色质沉淀分析

µCi/µl, specific activity 3000 Ci/mmol) (for hot-stop PCR [Steps 1-22] or SSCP [Steps 23-37] only)dNTPs (stock solutions at 25 mM for each dNTP)DNA loading buffer (6X)30% (v/v) glycerol0.25% (w/v) bromophenol blue0.25% (w/v) xylene cyanol FFStore