Sartorius赛多利斯VS2031VIVASPIN 20

Reverse Transcription of RNA- 20l rxn

. This is the quivalent of ~ 25-50 ug total RNA. (X-H uses much less). I have seen consistently better results with oligo dT primed RNA even for very long RNA where 5 prime regions are to be amplified. Many people swear by superscript in our experience biolabs

RNA Purification Protocol for 10-20mg of Animal Tissue

设备   1. Microcentrifuge capable of 13,000 x g 2. Sterile 1.5 mL microcentrifuge tubes 3. Ice bath 4. Microfuge tube pestle 实验步骤   1. Dissect tissue quickly and freeze in liquid nitrogen. Storage at -70°C

SQ Blood Mini Protocol for 50ul Clotted Blood

实验试剂   Regents to be supplied by user 1. Proteinase K (20mg/ml) 2. Isopropanol 3. 70% ethanol 实验设备   Equipments to be supplied by user 1. Microcentrifuge capable of 14,000 x g