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货号：T_70110-269-67C
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英文名称：FLASK 100ML COMPLETE 19/38
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文献和实验Replication timing by density transfer
, then add Pronase (0.01-0.05 mg/ml final conc.). [You can add dry Pronase. I usually dissolve the Pronase in 5 ml of minimal medium, just to avoid having the dry Pronase stick to the sides of the flask.] 5. Hold at 37o C for 90-120 min
Construction and Characterization of Adenovirus Vectors
the cells from the dish into the medium. Transfer to a 4-mL cryovial. x. Add 40% sucrose in PBAd to a final concentration of 4%. Vortex briefly. Use immediately or store at -80°C until use. xi
Procedures for In Vitro Production of Bovine Embryos
hood 100 ml pipet tips and pipettor OMM + supplements Mineral oil 60 x 15 petri dishes Plastic pasteur pipet 3-4 bottles of OCM + supplement (warmed @ 38.5°C) 1 L saline + Pen/Strep (warmed @ 38.5°C)Preparation for Oocyte Collection Scalpel Petri dishes
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