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货号：T_70110-269-67A
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英文名称：FLASK 25ML COMPLETE 19/38
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文献和实验Construction and Characterization of Adenovirus Vectors
as described above or in suspension. Grow the cells in suspension in 1X maintenance medium in suspension flasks, agitated at 70 rpm. When they reach a density of 5 x 105 cells/mL, dilute 1:2 or 1:3 with 1X maintenance medium. CsCl (cesium chloride
Replication timing by density transfer
, leu2-3,112, ura3-52, his6 in an A364A background. I aim for about 32 x 106 cells per time point. That gives enough DNA for at least 3 blots, leaving enough leeway for errors. 2. When the cell density is 2 x 106 cells/ml (OD660 ~ 0.16
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
7 mL of 1.15X ligation buffer. 19. Add 400 µL of 20% Triton X-100 (to a final concentration of 1%). Incubate with occasional gentle shaking for 1 h at 37°C. 20. Add 50 µL of T4 DNA ligase. Incubate for 4 h at 16°C
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