赛默飞世尔Thermo Fisher Comb 1.0mm

Loss of Heterozygosity

with a pipette. Insert the teeth of the comb approximately 1 mm into the gel. Pre-heat the gel at 1700 volts for 15-20 minutes. F: Gel Loading Remove the samples from the freezer. Denature the samples in a thermal cycler at 95°C for 5 mins. Remove

SDS-PAGE

for routine analytical gels, and 1.5 mm spacers to run more sample. I usually use a comb that makes 20 wells.2. Solutions:I. 1.5 M Tris-HCl, pH 8.8II. 1 M Tris-HCl, pH 6.8III. 30% acrylamide/bisacrylamide (37.5:1). This solution can be bought commercially

The ribonuclease protection assay (RPA)

P]UTP 1 µl T7 RNA polymerase (Keep at -20°C until use, return to -20°C immediately). Mix by gentle pipetting or flicking and quick spin in a microfuge. Incubate at 37°C for 1 hour. 2. Terminate the reaction by adding 2 µl of DNase. Mix