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- 文献和实验
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999
- 供应商:
北京泽平
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EA
货号:T_70312692825
中文名称:
英文名称:Immersion PC300 with bridge, Temperature: 300 ° C
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文献和实验Characterization of 5‐HT1A,B and 5‐HT2A,C Serotonin Receptor Binding
(Amersham Pharmacia Biotech, 222 to 225 Ci/mmol) or [3 H]p ‐MPPF (NEN) 170 mM Tris⋅Cl buffer, pH 7.6 ( appendix 2A ), room temperature and 4°C (adjust pH at desired temperature
A quick RNA mini-prep for Neurospora mycelial cultures
temperature. [E buffer: 50 mM Tris-Cl pH 8.0, 300 mM NaCl, 5 mM EDTA, pH 8.0, 2 SDS; autoclave and add 1 mM ATA and 14 mM ß-mercaptoethanol. ATA=aurintricarboxylic acid, ammonium salt (Sigma #A0885, St. Louis, MO)] 5. Thaw the powder in E buffer in 42°C
Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
is incubated for 35 thermal cycles as follows: 95°C for 45 seconds 55°C for 30 seconds (lower temperature for primers less than 20nt) 72°C for 1 minute f. 75 µl of H20 is added to the reaction below the oil and 90 - 95 µl of the diluted reaction
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